2016 Detection of HIT antibody dependent platelet aggregation using novel surface imprinting approach

Authors: Hussain M, Northoff H, Gehring FK

Journal: Talanta, 2016, 147, 1-7. doi: 10.1016/j.talanta.2015.09.027 

Institute: 

Biosensor Research Group, Institute of Clinical and Experimental Transfusion Medicine and Centre of Clinical Transfusion Medicine (ZKT), Tuebingen University and German Red Cross Blood Transfusion Service BW/H, Germany

Abstract: We present a fast, robust and straightforward spin force assisted surface imprinting approach for activated platelets and demonstrate that Heparin induced thrombocytopenia (HIT) platelet aggregation can be measured by this approach. A critical and challenging step in functional assays for HIT is platelet separation from the healthy donor's platelet-rich plasma (PRP). Our approach using surface imprinted polymer (MIP) for measurements on a quartz crystal microbalance with dissipation (QCM-D) enables monitoring of platelet aggregation directly in PRP thus eliminating the challenge of platelet separation. This is the first report of platelet imprinting. We also provide proof of principle that QCM-D technology can be applied for functional measurements of HIT antibodies.