2018 Multifunctional Biomaterial Coating Based on Bio-Inspired Polyphosphate and Lysozyme Supramolecular Nanofilm

Xinyuan Xu, Dongyue Zhang, Shangwei Gao, Toshikazu Shiba, Quan Yuan, Kai Cheng, Hong Tan, Jianshu Li

Biomacromolecules 2018, 19, 6, 1979-1989; DOI: 10.1021/acs.biomac.8b00002


College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China

Regenetiss Inc., 1-7-20, Higashi, Kunitachi, Tokyo 186-0002, Japan

State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China


Current implant materials have widespread clinical applications together with some disadvantages, the majority of which are the ease with which infections are induced and difficulty in exhibiting biocompatibility. For the efficient improvement of their properties, the development of interface multifunctional modification in a simple, universal, and environmently benign approach becomes a critical challenge and has acquired the attention of numerous scientists. In this study, a lysozyme-polyphosphate composite coating was fabricated for titanium(Ti)-based biomaterial to obtain a multifunctional surface. This coating was easily formed by sequentially soaking the substrate in reduced-lysozyme and polyphosphate solution. Such a composite coating has shown predominant antibacterial activity against Gram-negative bacteria (E. coli) and improved cell adhesion, proliferation, and differentiation, which are much better than those of the pure substrate. This facile modification endows the biomaterial with anti-infective and potential bone-regenerative performance for clinical applications of biomaterial implants.